1.Microsc Res Tech. 2000 Aug 1;50(3):184-95.
2.Methods Mol Biol. 2021;2248:271-279.
Tumour Necrosis Factor α (TNFα), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signalling events within cells, leading to necrosis or apoptosis. The protein is also important for resistance to infection and cancers. In view of these facts, TNF-α has been recommended as an important target for discovering drugs for autoimmune and inflammatory diseases and cancer.
Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 30pg/ml.
1μg (R: reducing condition, N: non-reducing condition).