Phe22-Asp839, with C-terminal 10*His FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDGGGSGGGSHHHHHHHHHH
100-120kDa
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
1. Bj?rbaek C, Uotani S, da Silva B, Flier JS. Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 1997;272:32686-32695.
Leptin cellular actions are mediated by leptin receptors (OB-Rs, Leptin R), which are membrane-anchored molecules binding to leptin and transducing its intracellular signal. Leptin R is encoded by the db gene. Leptin R is a member of cytokine receptor family that including various interleukins, interferon-γ, erythropoietin, growth hormone and prolactin. Proteolytic cleavage of transmembrane Leptin R produces a soluble leptin binding protein called soluble leptin receptor (sOB-R), which constitutes the major binding protein of circulating leptin in human plasma. The sOB-R exerts high affinity in binding leptin, antagonizing the membrane leptin receptors and, functionally, inactivating leptin. By binding circulating leptin, sOB-R modulates the bioavailability and efficacy of leptin, exerting a predominantly inhibitory effect on leptin signal transduction; thus, altering the sensitivity of target tissues to leptin actions and producing a state called leptin resistance in analogy to in sulin resistance. Genetic deficiency of either leptin or Leptin R causes severe obesity and obesity-associated diabetes.
2μg (R: reducing conditions, N: non-reducing conditions).
Immobilized Leptin, Human (Cat. No. UA040148) at 10.0μg/mL (100μL/well) can bind Leptin R/CD295 His Tag, Human (Cat. No. UA010682) with EC50 of 0.96-1.29μg/mL.
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