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CD58/LFA-3 His Tag Protein, Human

CD58/LFA-3 His Tag Protein, Human

货号: UA010759
价格: 2520
规格: 100μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    CD58, LFA3, Ag3

  • Accession

    AAH05930.1

  • 表达序列

    Phe29-Arg215, with C-terminal His Tag

  • 表达宿主

    HEK293

  • 分子量

    33-55kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4.

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893. Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939. Crawford, K. et al. (2003) Blood 102:1745.

  • 稀释度

背景介绍

  • CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily. CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells.

  • 生物活性JSON

    • Immobilized CD58/LFA-3 His Tag, Human (Cat. No. UA010759) at 2.0μg/mL (100μL/well) can bind CD2/SRBC Fc Chimera, Cynomolgus (Cat. No. UA010735) with EC50 of 0.14-0.22 μg/mL.

    • Immobilized CD58/LFA-3 His Tag, Human (Cat. No. UA010759) at 2.0μg/mL (100μL/well) can bind CD2/SRBC Fc Chimera, Human (Cat. No. UA010766) with EC50 of 86.40-97.74 ng/mL.

  • 电泳JSON

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured CD58/LFA-3 His Tag, Human (Cat. No. UA010759), can bind CD2/SRBC Fc Chimera, Human (Cat. No. UA010766) with an affinity constant of 12.17 nM as determined in SPR assay.