您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
TNF-α Protein, Mouse

TNF-α Protein, Mouse

货号: UA040173
价格: 1210
规格: 10μg
介绍: -
其他: -
产品规格

  • 物种

    Mouse

  • 分子别名

    NFα蛋白;Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2

  • Accession

    P06804

  • 表达序列

    Asp89-Leu235

  • 表达宿主

    E.coli

  • 分子量

    17kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE and SEC-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50＜15pg/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM PB, 300mM NaCl, 2mM TCEP, pH7.5

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Microsc Res Tech. 2000 Aug 1;50(3):184-95.

    2.Methods Mol Biol. 2021;2248:271-279.

  • 稀释度

背景介绍

  • Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

  • 生物活性JSON

    • Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 15pg/ml.

  • 电泳JSON

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • >95% as determined by SEC-HPLC.