Glu31-Arg369 with Human IgG1 Fc at C-Terminus
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
1.Ide, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5062.
2.Gardai, S.J. et al. (2003) Cell 115:13.
3.Lundberg, P. et al. (2007) Biochem. Biophys. Res. Commun. 352:444.
Signal regulatory protein alpha (SIRP alpha, CD172a), also known as SHPS-1 and formerly MyD-1, is a ~90 kDa type I transmembrane glycoprotein in the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPs are paired receptors with similar extracellular domains but distinct C-termini and functions. The human SIRP alpha, consisting of 503 amino acids, includes a 342 amino acid extracellular domain with one V-type and two C1 type Ig domains, and three potential N-glycosylation sites. It features a 110 amino acid cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 upon phosphorylation. SIRP alpha exhibits over 40 polymorphisms, including the notable BIT (also known as SIRP alpha 2 or PTPNS). Engagement of SIRP alpha generally elicits a negative regulatory signal. It recognizes CD47 on aged erythrocytes, platelets, or xenogenic cells, promoting their clearance from circulation. In the lung, SIRP alpha's recognition of surfactants SP-A and SP-D can inhibit alveolar macrophage cytokine production. Additionally, the CD47-SIRP alpha interaction facilitates macrophage fusion during osteoclastogenesis.
1μg (R: reducing condition, N: non-reducing condition).
The purity of SIRP alphaV2/CD172a Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.
Immobilized CD47 His Tag Protein, Human (Cat. No. UA010014) at 2.0μg/mL (100μL/well) can bind SIRP alphaV2/CD172a Fc Chimera Protein, Human (Cat. No. UA011151) with EC50 of 10.50-16.63ng/mL.
您现在的位置：