Ser30-His272, with N-terminal His Tag
38-70 kDa (Reducing)
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
N Engl J Med. 2003 Dec 25;349(26):2483-94.
Bone. 2006 Oct;39(4):754-66. Epub 2006 May 26.
Dickkopf-1 (DKK-1) is a secreted protein that belongs to the Dickkopf family of Wnt signaling inhibitors. In mice, DKK-1 is expressed in various tissues, including bone, brain, and reproductive organs, and plays a critical role in regulating the Wnt signaling pathway. DKK-1 functions by binding to the Wnt co-receptor LRP5/6, thereby inhibiting the canonical Wnt signaling cascade. By antagonizing Wnt signaling, DKK-1 suppresses the differentiation and proliferation of osteoblasts, leading to the inhibition of bone formation. DKK-1 is also involved in other biological processes, such as embryonic development, tissue homeostasis, and cancer progression. Dysregulation of DKK-1 has been implicated in skeletal disorders, such as osteoporosis and osteoarthritis, as well as in various cancers, highlighting the importance of DKK-1 in development and disease.
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293T human embryonic kidney cells. Recombinant Mouse DKK-1 inhibits a constant dose of 50 ng/ml of Recombinant Human Wnt‑Surrogate. The EC50 for this effect is less than 90 ng/ml.
2μg (R: reducing condition, N: non-reducing condition).
>95% as determined by RP-HPLC.
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