Met1-Asp320 with his tag at C-Terminus
33 kDa (Reducing)
PBS, pH7.4
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Nat Rev Mol Cell Biol. 2004 Nov;5(11):897-907.
Annexin V is a calcium-dependent phospholipid-binding protein that has been widely used as a molecular probe for detecting and quantifying apoptotic cells. Annexin V has a high affinity for phosphatidylserine, a phospholipid that is normally located on the inner leaflet of the plasma membrane but becomes exposed on the outer surface of the membrane during the early stages of apoptosis. By binding to phosphatidylserine, Annexin V can be conjugated with fluorophores or other detection molecules to label apoptotic cells for flow cytometry, fluorescence microscopy, or other imaging techniques. The use of Annexin V in apoptosis assays has become a standard method for studying cell death processes in research and clinical settings.
2μg (R: reducing condition, N: non-reducing condition).
Immobilized Annexin V His Tag Protein, Human (Cat. No. UA011104) at 2.0μg/mL (100μL/well) can bind Annexin V Recombinant Rabbit mAb (S-1395-41) (Cat. No. S0B0871) with EC50 of 1.71-2.37ng/ mL.
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