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Human PD-L1 Standard for Quantitive Assays

Human PD-L1 Standard for Quantitive Assays

货号: UA010001-STD
价格: 1000
规格: 1Vial
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    Programmed cell death 1 ligand 1, CD274, B7-H1, Programmed death ligand 1

  • Accession

    Q9NZQ7

  • 表达序列

    Phe19-Arg238, with C-terminal His Tag

  • 表达宿主

    HEK293

  • 分子量

    30-35 KD(Reducing)

  • 纯度

    ＞95%, by SDS-PAGE under reducing conditions

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH 7.2, 5%海藻糖

  • 溶解方法

    Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 稀释度

背景介绍

  • 细胞程式死亡-配体1(Programmed cell death 1 ligand 1，PD-L1)是大小为 40kDa人类体内表达的第一型跨膜蛋白，据信其在某些特殊情形(例如怀孕、组织移植、自体免疫疾病，以及诸如肝炎等某些疾病)下，免疫系统的抑制有关。正常情形下免疫系统会对聚集在淋巴结或脾脏的外来抗原产生反应，促进抗原特异性的细胞毒杀性T细胞(CD8+ T cell)增生。而细胞程序化死亡受体-1(PD-1)与细胞程式死亡-配体1(PD-L1)结合，可以传导抑制性的信号，减低淋巴结CD8+ T细胞的增生，而且PD-1还可以借此调节Bcl-2基因，控制淋巴结中抗原特异性T细胞的聚积。本产品系由HEK293细胞株表达人源PD-L1，C-6His重组蛋白，经多步纯化后过滤、添加5%海藻糖、冻干制成。

  • 生物活性JSON

    • When PD-L1 His tag, Human is immobilized at 2.8µg/mL, it binds to PD-1 Fc Chimera, Human with an EC50 of 4.334-5.695µg/mL.
    • PD-L1 His Tag, Human (Cat. No.UA010001) captured on CM5 chip via anti-His antibody, can bind PD-1 Fc Chimera, Human (Cat. No. UA010002) with an affinity constant of 0.1836 μM as determined in a SPR assay (Biacore T200).
    • Loaded PD-1 Fc Chimera, Human (Cat. No. UA010002) on Protein A Biosensor, can bind PD-L1 His Tag, Human (Cat. No. UA010001) with an affinity constant of 1.3 μM as determined in BLI assay.

  • 电泳JSON

    • PD-L1 His Tag, Human，1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.

  • ELISA

    • Immobilized PD-L1 His Tag Protein, Human (Cat. No. UA010001) at 1.0μg/mL (100μL/well) can bind S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-119-286) (Cat. No. S0B2287) with EC50 of 0.89-1.21ng/mL.