Tyr37-Asp193
18 kDa (Reducing)
20mM Tris, 200mM NaCl, pH8.0
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
1. Acta Biochim Pol 2016;63(1):59-63. Epub 2016 Feb 17.
2. Int J Mol Sci 2019 Feb 2;20(3):649.
Interleukin 18 (IL-18) is a pleiotropic cytokine involved in the regulation of innate and acquired immune response. In the milieu of IL-12 or IL-15, IL-18 is a potent inducer of IFN-gamma in natural killer (NK) cells and CD4 T helper (Th) 1 lymphocytes. However, IL-18 also modulates Th2 and Th17 cell responses, as well as the activity of CD8 cytotoxic cells and neutrophils, in a host microenvironment-dependent manner. IL-18 is a cytokine that stimulates various cell types and has pleiotropic functions. Recently, IL-18 has also been shown to execute specific effects in pancreatic diseases, including acute and chronic pancreatitis, as well as pancreatic cancer.
Measured by its ability to enhance IFN-γ secretion in KG-1 human myelomonocyte cells. The EC50 for this effect is less than 10ng/ml.
1μg (R: reducing condition, N: non-reducing condition).
>95% as determined by RP-HPLC.
Measured by its binding ability in a functional ELISA. When IL-18Rβ/IL-1R7 His Tag, Human (Cat. No. UA010888) is immobilized at 2.0µg/mL (100 µL/well), in the present of IL-18, Human (Cat. No. UA040111), IL-18Rα/IL-1R5 Fc Chimera, Human (Cat. No. UA010874) binds with an ED50 of 8.62-11.50 ng/mL.
When IL-18Rβ/IL-1R7 Fc Chimera Protein, Human (Cat. No. UA010873) is immobilized at 2.0µg/mL (100 µL/well), in the present of IL-18, Human (Cat. No. UA040111), IL-18Rα/IL-1R5 His Tag Protein, Human (Cat. No. UA010914) binds with an ED50 of 40.10-49.63ng/mL.
Immobilized IL-18, Human (Cat. No. UA040111) at 2.0μg/mL (100μL/well) can bind IL-18BP Fc Chimera, Human (Cat. No. UA010587) with EC50 of 2.20-3.25 ng/mL
Immobilized IL-18, Human (Cat. No. UA040111) at 2.0μg/mL (100μL/well) can bind IL-18BP His Tag, Human (Cat. No. UA010574) with EC50 of 0.68-0.91 ng/mL.
Anti-His antibody Immobilized on CM5 Chip captured IL-18BP His Tag, Human (Cat. No. UA010574), can bind IL-18, Human Cat. No. UA040111) with an affinity constant of 3.15nM as determined in SPR assay.
CM5 Chip captured IL-18Rα/IL-1R5 His Tag, Human (Cat. No. UA010914), can bind IL-18, Human (Cat. No. UA040111) with an affinity constant of 1.20nM as determined in SPR assay.
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