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Biotinylated Transferrin His&Avi Tag Protein, Human

Biotinylated Transferrin His&Avi Tag Protein, Human

货号: UA011027
价格: 2800
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    Beta-1 metal-binding globulin，Siderophilin，Serotransferrin，TF，TRF，DKFZp781D0156, PRO1557, PRO2086，HEL-S-71p，TFQTL1

  • Accession

    P02787

  • 表达序列

    Val20-Pro698 with His & Avi Tag at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    70-85kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE & SEC-HPLC

  • 内毒素含量

    <1EU/μg

  • 活性

    Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The EC50 for this effect is typically 9-25ng/ml. Immobilized Biotinylated Transferrin His&Avi Tag Protein, Human (Cat. No. UA011027) at 2μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Human Transferrin R / CD71 Protein, Fc Tag with EC50 of 8.90-14.7ng/ml.

  • 标记

    Biotin

  • 标签

    Avi Tag, His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Stearne P.A. et al. (1985) J. Immunol. 134:3474.
    Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
    Skikne, B.S. (2008) Am. J. Hematol. 83:872.
    Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
    Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
    Radoshitzky, S.R. et al. (2007) Nature 446:92.

  • 稀释度

背景介绍

  • Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is believed to have originated from an ancient gene duplication event, resulting in homologous C and N-terminal domains that each bind one ferric ion. The primary function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to proliferating cells throughout the body. Additionally, transferrin may serve a physiological role as a granulocyte/pollens-binding protein (GPBP), which is involved in the clearance of certain organic materials and allergens from the serum.

  • 生物活性JSON

    • Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The EC50 for this effect is typically 9-25ng/ml.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • The purity of Biotinylated Transferrin His & Avi Tag Protein, Human is greater than 95% as determined by SEC-HPLC.

  • ELISA

    • Immobilized Biotinylated Transferrin His&Avi Tag Protein, Human (Cat. No. UA011027) at 2μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Human Transferrin R / CD71 Protein, Fc Tag with EC50 of 8.90-14.7ng/ml.