His25-Gly103 with His Tag at the C-Terminus
10-15kDa (Reducing)
PBS, PH7.4, 5% trehalose
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
1. Shouyan Deng, et al. The ITPRIPL1- CD3ε axis: a novel immune checkpoint controlling T cells activation. bioRxiv, 2022.
2. Zhong, X. and Zhong, G. Prognostic biomarker identification and tumor classification in breast cancer patients by methylation and transcriptome analysis. FEBS Open Bio, 2021.
Inositol 1,4,5-trisphosphate Receptor Interacting Protein-Like 1 (ITPRIPL1) is a transmembrane protein that is widely expressed in the testis and is associated with immune evasion mechanisms. ITPRIPL1 is specifically expressed in immune-privileged tissues, 'hot' tumors lacking PD-L1 expression, and tumors that do not respond to PD-1 blockade therapy. Experimental evidence has demonstrated that ITPRIPL1 can impair T cell function both in vitro and in vivo through interactions with CD3e, with a negative correlation observed between ITPRIPL1 expression levels in antigen-presenting cells and T cell cytotoxicity.
In the context of breast cancer, ITPRIPL1 has been identified as a hypermethylated, low-expression gene. Furthermore, using bioinformatics methods, ITPRIPL1 has been established as an independent biomarker for the prognosis of lung adenocarcinoma. Therefore, targeting the ITPRIPL1-CD3e axis, particularly in patients with PD-1/PD-L1 negative tumors, represents a promising therapeutic strategy to mitigate immune evasion.
ITPRIPL1 is also a significant biomarker for non-small cell lung cancer (NSCLC). The detection of ITPRIPL1 in tumors can aid in improved diagnosis, reveal the infiltration of CD8 positive T cells, monitor disease progression, and predict prognosis.
2μg (R: reducing condition, N: non-reducing condition).
The purity of ITPRIPL1 His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
Immobilized ITPRIPL1 His Tag Protein, Human (Cat. No. UA011092) at 2.0μg/mL (100μL/well) can bind CD3L1 (ITPRIPL1) Recombinant Rabbit mAb (S-1716-50) (Cat. No. S0B1227) with EC50 of 0.87-1.24 ng/ mL.
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