D551-T919，N-terminal 8x His and SUMO tag, C-terminal Myc tag
56.5 kDa
25mM Hepes, 150 mM NaCl, 2 mM DTT, 10 μM DHT, 5% glycerol, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The ANDR(LBD) activity was detected using HTRF binding assay. The reaction was performed by incubating the ANDR(LBD) protein, CRBN/DDB1 and ARV-110, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 1 μg ANDR(LBD)
2 3 μg ANDR(LBD)
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