Trp42-Ala771 with His tag at the C-Terminus
100-120kDa (Reducing)
PBS, pH7.4, 5% trehalose
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
1.Weller A, Isenmann S, Vestweber D. Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. J Biol Chem. 1992 Jul 25;267(21):15176-83.
Mouse P-Selectin, alternatively known as GMP-140, LECAM-3, PADGEM, or CD62P, belongs to the Selectin family of cell surface glycoproteins. It is expressed by activated platelets and endothelial cells. The Mouse P-Selectin cDNA encodes a 768 amino acid (aa) type I transmembrane protein, which includes a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain, and a short (35 aa) cytoplasmic domain.
The extracellular domain of Mouse P-Selectin contains an NH2-terminal C-type lectin domain and an EGF-like domain, followed by a series of complement factor A repeat homology domains. It shares approximately 73% sequence homology with the extracellular domains of human P-Selectin.
Mouse P-Selectin plays a critical role in the adhesion of leukocytes and neutrophils to the endothelium. Together with L-Selectin, it initiates the interaction between circulating leukocytes and endothelial cells, leading to the characteristic 'rolling' of leukocytes along the endothelium. This initial interaction is followed by the formation of a stronger bond involving E-Selectin, and subsequently ICAM-1 and VCAM-1. This sequence of molecular interactions facilitates the extravasation of white blood cells through the blood vessel wall and into the extracellular matrix of surrounding tissues.
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The EC50 for this effect is typically 60-90ng/ml.
2μg (R: reducing condition, N: non-reducing condition).
The purity of P-Selectin/CD62P His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
The purity of P-Selectin/CD62P His Tag Protein, Human is greater than 95% as determined by RP-HPLC.
Immobilized P-Selectin/CD62P His Tag Protein, Human (Cat. No. UA011083) at 2.0μg/mL (100μL/well) can bind Inclacumab (Cat. No. UA011071) with EC50 of 2.08-2.61ng/mL.
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