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P-Selectin/CD62P Fc Chimera Protein, Mouse

P-Selectin/CD62P Fc Chimera Protein, Mouse

货号: UA011067
价格: 1280
规格: 25μg
介绍: -
其他: -
产品规格
  • 物种

    Mouse
  • 分子别名

    GMP-140, LECAM3, PADGEM
  • Accession

    Q01102
  • 表达序列

    Trp42-Ala709, with C-terminal Fc
  • 表达宿主

    HEK293
  • 分子量

    115-140kDa (Reducing)
  • 纯度

    >95% by SDS-PAGE & SEC-HPLC
  • 活性

    Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The EC50 for this effect is less than 150ng/ml.
  • 标记

    Unconjugated
  • 标签

    Human Fc Tag
  • 性状

    Lyophilized Powder
  • 缓冲体系

    PBS, PH7.4, 5% trehalose
  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.
  • 文献引用

    Weller A, Isenmann S, Vestweber D. Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. J Biol Chem. 1992 Jul 25;267(21):15176-83.

  • 稀释度

背景介绍
  • Mouse P-Selectin, also known as GMP-140, LECAM-3, PADGEM, or CD62P, is a member of the Selectin family of cell surface glycoproteins. It is expressed by activated platelets and endothelial cells. The cDNA for Mouse P-Selectin encodes a 768 amino acid (aa) type I transmembrane protein. This protein comprises a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain, and a short (35 aa) cytoplasmic domain.
    The extracellular domain of Mouse P-Selectin features an NH2-terminal C-type lectin domain and an EGF-like domain, succeeded by a series of complement factor A repeat homology domains. There is approximately 73% sequence homology between the extracellular domains of human and mouse P-Selectin.
    P-Selectin is crucial for the adhesion of leukocytes and neutrophils to the endothelium. In conjunction with L-Selectin, it mediates the initial interaction between circulating leukocytes and endothelial cells, resulting in a characteristic 'rolling' phenomenon of leukocytes along the endothelium. Following this initial interaction, a stronger bond forms, involving E-Selectin, and subsequently ICAM-1 and VCAM-1. This sequence of events ultimately facilitates the extravasation of white blood cells through the blood vessel wall and into the surrounding tissue matrix.

  • 生物活性JSON

    • Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The EC50 for this effect is less than 150ng/ml.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • The purity of P-Selectin/CD62P Fc Chimera Protein, Mouse is more than 95% determined by SEC-HPLC.