R669-A1210(end), d746-750, T790M, C797S
RRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKTSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGSLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
87.2 kDa
50 mM Hepes, 150 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt.
And avoid repeated freeze-thaws cycles.
EGFR[d746-750/T790M/C797S] titration and inhibitor IC50 determination
The EGFR[d746-750/T790M/C797S] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[d746-750/T790M/C797S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 1 μg EGFR[d746-750/T790M/C797S]
2 3 μg EGFR[d746-750/T790M/C797S]
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