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70 kD
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Store at -25 ~ -15℃ for 2 years
[1] Nicholson AW. Ribonuclease III mechanisms of double-stranded RNA cleavage. Wiley Interdiscip Rev RNA. 2014 Jan-Feb;5(1):31-48. [2] Wu CX, Xu XJ, Zheng K, Liu F, Yang XD, Chen CF, Chen HC, Liu ZF. Characterization of ribonuclease III from Brucella. Gene. 2016 Apr 1;579(2):183-92. [3] Sun, Weimei et al. Catalytic mechanism of Escherichia coli ribonuclease III: kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis. Nucleic Acids Research 33 (2005): 807 - 815.
The processing of double-stranded(ds) RNA by RNase III family members is an essential step in the maturation and decay of coding and noncoding RNAs, including miRNAs and siRNAs. RNase III family members share a unique fold (RNase III domain) that can dimerize to form a structure that binds dsRNA and cleaves phosphodiesters on each strand, providing the characteristic 2 nt, 3'-overhang product ends. RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA).
Storage Solution: 2U/μl RNase Ⅲ、500 mM NaCl、10 mM Tris-HCl、0.5 mM EDTA、1 mM DTT、50% Glycerol（pH 8 @ 25°C） 10*Reaction Buffer: 500 mM Tris-HCl、1 mM DTT 50 mM NaCl (pH 7.5 @ 25°C) 10*EDTA: 500 mM 10*MnCl2: 200 mM
Set-up a typical reaction as follows 1）Add the following components in sequence 2）Incubate at 37°C for 20 minutes This reaction can be scaled up according to experimental needs.
Please avoid repeated freeze-thaw cycles.
Marker（100 bp~2000 bp）：1 ul
Lane1、Negative Control (The mixture substrate of Double-stranded RNA and Single-stranded RNA)
Lane2、Mixture substrate + Competitive product RNase III 1 U
Lane3、Mixture substrate + Competitive product RNase III 2 U
Lane4、Mixture substrate + Competitive product RNase III 4 U
Lane 5、Mixture substrate + UA RNase III 1 U
Lane 6、Mixture substrate + UA RNase III 2 U
Lane 7、Mixture substrate + UA RNase III 4 U
1μg (R: reducing condition, N: non-reducing condition).
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