A2-S659(end)
101876.0
-80℃
The BTK[T474S] activity was detected using HTRF technology. The reaction was performed by incubating the BTK[T474S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 1 μg BTK[T474S]
2 3 μg BTK[T474S]