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CDK12/Cyclin K Protein

CDK12/Cyclin K Protein

货号: UA080053
价格: 3835
规格: 10μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • Accession

    Q9NYV4-1, O75909-3

  • 表达序列

    CDK12: Q696-S1082

    QPYKKRPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSGVVVEEPPPSKTSRKETTSGTSTEPVKNS

    Cyclin K:  M1-S300

    MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPS

  • 表达宿主

    Baculovirus-InsectCells

  • 分子量

    CDK12: 61.7 kDa; Cyclin K: 48.1 kDa

  • 纯度

    >85% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 活性

    ADP-Glo_Function

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione , 1 mM DTT, 0.05% Brij35, 10% glycerol, pH 7.5

  • 储存条件

    -80℃

  • 稀释度

  • 生物活性JSON

    • CDK12/Cyclin K titration in ADP-Glo assay

    • The CDK12/Cyclin K activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK12/Cyclin K protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.

  • 电泳JSON

  • 体积排阻色谱JSON(SEC-HPLC)