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Nectin-4 His Tag Protein, Human

Nectin-4 His Tag Protein, Human

货号: UA010020
价格: 1800
规格: 50μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR; Nectin4

  • Accession

    Q96NY8-1

  • 表达序列

    Q96NY8-1, Gly32-Ser349, with C-8*His GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASGGGSGGGSHHHHHHHH

  • 表达宿主

    HEK293

  • 分子量

    44-47 kDa(reducing)

  • 纯度

    ＞95%, by SDS-PAGE under reducing conditions

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 稀释度

背景介绍
  • Nectin-4, also known as poliovirus-like receptor 4 (PVRL4), is a newly discovered cell adhesion molecule, which belongs to the Nectin family. There are four members of the Nectin family: Nectin-1, Nectin-2, Nectin-3 and Nectin-4. There are three continuous immunoglobulin-like domains outside the cells of Nectin protein, which are N-terminal Ig-V domain and C-terminal Ig-C2 domain. Nectin-4 is mainly expressed in placenta, expressed in normal human tissues including skin, bladder, salivary glands, esophagus, breast and stomach, and abnormally expressed on the surface of a variety of cancer cells such as breast cancer, bladder cancer, non-small cell lung cancer and pancreatic cancer. Nectin-4 promotes the proliferation, differentiation, migration and invasion of tumor cells by activating PI3K/Akt pathway. In some cancers, such as urothelial carcinoma, the high expression of Nectin-4 can promote tumor cell proliferation and angiogenesis and reduce cancer cell apoptosis. Some studies have shown that Nectin-4, as a new ligand of TIGIT, can inhibit the activity of natural killer cells. These suggest that Nectin-4 may be a potential target for cancer therapy.

  • 生物活性JSON

    • Immobilized Nectin-4 His Tag, Human (Cat. No. UA010020) at 2μg/mL (100μL/well) can bind Anti-Human Nectin-4 Monoclonal Antibody with EC50 of 1.70-2.16 ng/ml. 

  • 电泳JSON

    • Nectin-4 His Tag, Human，2μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured Nectin-4 His Tag, Human (Cat. No. UA010020), can bind Nectin-1 Fc Chimera, Human (Cat. No. UA010016) with an affinity constant of 0.521μM as determined in SPR assay.