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Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-510-64)

Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-510-64)

货号: S0B0363
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Akt (Ser473)

  • 分子别名

    RAC-alpha serine/threonine-protein kinase, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC, AKT1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane, Cytoplasm, Nucleus

  • Accession

    P31749, Q9Y243, P31751

  • 克隆号

    S-510-64

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    Dot blot, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
Dot bolt 1:1000
IP 1:50
背景介绍

  • Akt or Protein kinase B, is a serine/threonine kinase that plays an important role in regulating a number of cellular processes such as growth, metabolism and survival. The importance of the Akt pathway is highlighted by the mutation of various components of the pathway in human cancers such as the PTEN and PI3-kinase (P110α), which occur in more than 30% of human tumors. For Akt to achieve full activation, phosphorylation is needed at both serine 473 (ser473) of the hydrophobic tail and threonine 308 (thr308) of the activation motif, upon growth factor ligation to the receptor tyrosine kinases.

  • 免疫印迹

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HeLa starve overnight, then treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: C6 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 免疫沉淀

    • Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg (Input)
      Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 斑点杂交

    • Dot blot result of Phospho-Akt (Ser473) Rabbit mAb 
      Lane 1: phospho-Akt (Ser473) peptide
      Lane 2: Akt peptide
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution