您现在的位置: 首页   -  产品中心   -  免疫学   -   抗体
FITC-Labeled CD19 Fc Chimera Protein, Human

FITC-Labeled CD19 Fc Chimera Protein, Human

货号: UA010211
价格: 3400
规格: 50μg
介绍: -
其他: -
产品规格
  • 物种

    Human
  • Accession

    P15391-1
  • 表达序列

    Amino acid sequence(Pro20-Lys291, with C-terminal human IgG Fc)

    PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKAAAPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

  • 表达宿主

    CHO
  • 分子量

    80 kDa,150kDa(Reducing)

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg
  • 标记

    FITC
  • 标签

    His Tag
  • 性状

    Liquid
  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 稀释度

背景介绍
  • CD19 is a type-I transmembrane glycoprotein of 95 kDa that belongs to the immunoglobulin superfamily and is widely expressed on B cells throughout most stages of B-cell differentiation, though its expression is down-regulated during their terminal differentiation to plasma cells. CD19 maps to chromosome 16p11.2, where it encodes a 540 amino acid protein with two extracellular C-type IgSF domains as well as a large, approximately 240 residue, cytoplasmic tail that exhibits extensive conservation between mice and humans. CD19 is a signal amplifying coreceptor whose expression is restricted to B cells and follicular dendritic cells.  CD19 exists in a multimolecular complex with CD21 (complement receptor type 2, CR2), the tetraspanin CD81 and CD225 on the B cell surface. Via its interaction with CD21, CD19 serves as a signal transducing device for complement-conjugated antigen in B cells. Coligation of the BCR and the CD19/CD21 complex lowers the threshold for B cell activation by approximately two orders of magnitude. CD19 is a potential target for monoclonal antibody therapy, and preliminary data have demonstrated its effectiveness in B-cell depletion, making this an attractive therapy for autoimmune disorders and treatment of malignant B-cell lymphomas.CD19 serves as an attractive target for immunotherapy for many reasons: (1) It is expressed on most B-cell malignancies; (2) it is expressed in the B-cell lineage at an early stage, even before the expression of CD20; (3) it internalizes efficiently in lymphoma tumor models252; and (4) it is possibly involved in the development of B-cell cancers.

  • 流式分析

    • 2e5 of transient transfected anti-CD19 ScFv CAR-293 cells were stained with 0.1ug FITC - CD19 Fc Chimera Protein, Human, (Cat. No. UA010211) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). FITC signal was used to evaluate the binding activity.

      2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with 0.01ug CD19 Fc Chimera Protein, Human (Cat. No. UA010042) (Fig. D). Alexa Fluor 647 signal was used to evaluate the binding activity.

      2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with isotype and G4S Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.

  • 生物活性JSON

    • Immobilized Anti-CD19 Antibody, Mouse Monoclonal at 0.5μg/mL (100μL/well) can bind FITC-Labeled CD19 Fc Chimera, Human (Cat. No. UA010211) with EC50 of 11.12ng/ml.
    • Immobilized Anti-CD19 Antibody, Mouse Monoclonal at 2μg/mL (100μL/well) can bind FITC-Labeled CD19 Fc Chimera, Human (Cat. No. UA010211) with EC50 of 0.43μg/ml.
  • 电泳JSON

    • 1μg (R: reducing conditions).