Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate)

ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody (S0B0102) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat anti-Mouse IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

IF shows positive staining in paraffin-embedded human tonsil. Anti-PD-1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4008) was used as secondary antibody at 1/500 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

Negative control: IF shows negative staining in paraffin-embedded human liver. Anti-PD-1 antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4008) was used as secondary antibody at 1/500 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.