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Stat1 Recombinant Rabbit mAb (S-559-91)

Stat1 Recombinant Rabbit mAb (S-559-91)

货号: S0B0358
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Stat1

  • 分子别名

    Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84

  • 细胞定位

    Cytoplasm, Nucleus

  • 克隆号

    S-559-91

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20°C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
背景介绍

  • Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. It is a member of the STAT protein family. All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Interleukin 6 (IL-6), or IL-27.

  • 免疫印迹

    • WB result of Stat1 Rabbit mAb
      Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 5 µg
      Lane 2: HeLa whole cell lysate 5 µg
      Lane 3: MCF7 whole cell lysate 5 µg
      Lane 4: A549 whole cell lysate 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 87 kDa
      Observed MW: 84, 91 kDa

    • WB result of Stat1 Rabbit mAb
      Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
      Lane 1: mouse heart lysate 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 87 kDa
      Observed MW: 84, 91 kDa

    • WB result of Stat1 Rabbit mAb
      Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
      Lane 1: rat heart lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 87 kDa
      Observed MW: 91 kDa

  • 免疫沉淀

    • Stat1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Stat1 in 0.4 mg 293T whole cell lysate.
      Western blot was performed on the immunoprecipitate using Stat1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: 293T whole cell lysate 20 µg (Input)
      Lane 2: Stat1 Rabbit mAb IP in 293T whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
      Predicted MW: 87 kDa
      Observed MW: 84, 91 kDa