12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:5000-1:10000 |
| ICC | 1:500 |
V5-tag is a short peptide tag for detection and purification of proteins. The V5-tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.
WB result of V5 Tag Mouse mAb (S-610-66)
Lysate: V5 tagged fusion protein 20 ng
Primary antibody:
Lane 1: V5 Tag Mouse mAb at 1/5000 dilution
Lane 2: V5 Tag Mouse mAb at 1/10000 dilution
Lane 3: V5 Tag Mouse mAb at 1/50000 dilution
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
ICC shows positive staining in Histone H3-Flag-V5-transfected 293T cells. Anti-V5 Tag antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
Negative control: ICC shows negative staining in vector-transfected 293T cells. Anti-V5 Tag antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
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