12 months from date of receipt / reconstitution, 4 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| IF | 1:200 |
| FCM | 1:200 |
CD8α encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Flow cytometric analysis of mouse primary splenocytes labeling CD8α (Alexa Fluor® 647 Conjugate) antibody at 1/200 (1 μg) dilution / (right panel) compared with a Rabbit IgG, Isotype Control / (left panel). Cells were surface stained with CD4-APC-Cy7, then stained with Rabbit IgG (Left) / CD8α (Right) separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
IF shows positive staining in paraffin-embedded mouse thymus. Anti- CD8α (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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