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RNA polymerase II CTD repeat YSPTSPS (phospho S7) Recombinant Rat mAb (S-R198)

RNA polymerase II CTD repeat YSPTSPS (phospho S7) Recombinant Rat mAb (S-R198)

货号: S0B0285
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rat

  • 抗原名称

    RNA polymerase II CTD repeat YSPTSPS (phosphor S7)

  • 分子别名

    DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2A

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P24928

  • 克隆号

    S-R198

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG1

  • 应用

    ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein G

  • 浓度

    0.4 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:4000
ICC 1:100
IP 1:40
背景介绍

  • RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. The purified enzyme has typically 10–12 subunits (12 in humans and yeast). The subunit RPB1 contains a carboxy terminal domain (CTD) composed of up to 52 heptapeptide repeats (YSPTSPS) that are essential for polymerase activity.

  • 免疫印迹

    • WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb at 1/4000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: 293T whole cell lysate 20 µg Lane 3: HeLa whole cell lysate (phosphatase treated) 20 µg Lane 4: 293T whole cell lysate (phosphatase treated) 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 217 kDa Observed MW: 260 kDa

    • WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S7) Rat mAb at 1/4000 dilution Lane 1: RAW 264.7 whole cell lysate 20 µg Lane 2: C2C12 whole cell lysate 20 µg Lane 3: RAW264.7 whole cell lysate (phosphatase treated) 20 µg Lane 4: C2C12 whole cell lysate (phosphatase treated) 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 217 kDa Observed MW: 260 kDa

  • 免疫沉淀

    • RNA polymerase II CTD repeat YSPTSPS (phosphor S7) Rat mAb at 1/40 dilution (1 µg) immunoprecipitating RNA polymerase II CTD repeat YSPTSPS (phosphor S7) in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using RNA polymerase II CTD repeat YSPTSPS (phosphor S7) Rat mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/10000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: RNA polymerase II CTD repeat YSPTSPS (phosphor S7) Rat mAb IP in HeLa whole cell lysate
      Lane 3: Rat monoclonal IgG1 IP in HeLa whole cell lysate
      Predicted MW: 217 kDa
      Observed MW: 260 kDa

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S7) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.