M1-P509(end)
-80℃
LCK titration in HTRF Assay
The LCK activity was detected using HTRF technology. The reaction was performed by incubating the LCK protein , ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
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