12 months from date of receipt / reconstitution, 4 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| FCM | 1:2000 |
| ICC | 1:200 |
| IF | 1:200 |
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 also functions in cell adhesion, signal transduction and calcium signaling. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications including social amnesia possibly related to autism. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism, as well as some genetically determined conditions.
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / Ramos (Human Burkitt's lymphoma B lymphocyte, right) cells labelling CD38 (Alexa Fluor 594 Conjugate) antibody at 1/2000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: HeLa
ICC shows positive staining in Ramos cells. Anti-CD38 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in HeLa cells. Anti-CD38 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD38 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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