| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:50 |
Mixed lineage kinase domain-like (MLKL) is the executioner in the caspase-independent form of programmed cell death called necroptosis. Receptor-interacting serine/threonine protein kinase 3 (RIPK3) phosphorylates MLKL, triggering MLKL oligomerization, membrane translocation and membrane disruption [PMID: 34698396].
WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: HUVEC whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Lane 4: HeLa whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa
WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: RAW 264.7 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa
WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: C6 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa
ICC shows positive staining in HT-29 cells. Anti-MLKL antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
Negative control: ICC shows negative staining in Raji cells. Anti-MLKL antibody was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI
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