PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
WB result of Rabbit IgG Isotype Control Primary antibody: Rabbit IgG Isotype Control at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Rabbit IgG Isotype Control Primary antibody: Rabbit IgG Isotype Control at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Lane 2: mouse spleen lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Rabbit IgG Isotype Control Primary antibody: Rabbit IgG Isotype Control at 1/1000 dilution Lane 1: C6 whole cell lysate 20 µg Lane 2: rat spleen lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Rabbit IgG-isotype control at 1/50 dilution (1 μg)/ (Red) (left panel) / Histone H3 antibody at 1/500 (0.1 μg) dilution (S0B0079) / (red) (Right panel) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG Alexa Fluor 488 was used as the secondary antibody.
Flow cytometric analysis of mouse primary splenocytes labeling Rabbit IgG-isotype control at 1/50 dilution (1 μg) / (left panel) compared with CD8α antibody at 1/50 (1 μg) dilution (S0B0034) / (right panel). Goat Anti-Rabbit IgG Alexa Fluor 488 was used as the secondary antibody. Then cells were stained with CD4 Alexa Fluor 647 separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
Atg7 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Atg7 in 0.4 mg Jurkat whole cell lysate.
Western blot was performed on the immunoprecipitate using Atg7 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: Jurkat whole cell lysate 20 µg (Input)
Lane 2: Atg7 Rabbit mAb IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
Predicted MW: 77 kDa
Observed MW: 75 kDa
IHC shows negative staining in paraffin-embedded human liver. Rabbit mAb IgG Isotype Control was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human spleen. Rabbit mAb IgG Isotype Control was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human tonsil. Rabbit mAb IgG Isotype Control was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Rabbit mAb IgG Isotype Control was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded rat liver. Rabbit mAb IgG Isotype Control was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows negative staining in HeLa cells. Rabbit mAb IgG Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
ICC shows negative staining in NIH/3T3 cells. Rabbit mAb IgG Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
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