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NY-ESO-1 Recombinant Rabbit mAb (SDT-R154)

NY-ESO-1 Recombinant Rabbit mAb (SDT-R154)

货号: S0B2206
价格: 700
规格: 25ul
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    NY-ESO-1
  • 分子别名

    Cancer/testis antigen 1, CTAG1B, CT6.1, CTAG, CTAG1, ESO1, LAGE2A, LAGE2B, LAGE-2, CTAG1A, LAGE2
  • 免疫原

    N/A
  • 细胞定位

    Cytoplasm
  • Accession

    P78358
  • 克隆号

    SDT-R154
  • 抗体类型

    Recombinant mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
背景介绍
  • New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies [PMID: 25412843]. It is a protein consisting of 180 amino acids, and its gene is located in the Xq28 region of the X chromosome. As a member of the cancer testis antigen (CTA) family, NY-ESO-1 has been shown to be expressed in spermatogonia, primary spermatocytes, oogonia, and placenta and in a variety of cancers, such as melanoma, ovarian cancer, cervical cancer [PMID: 28025978, PMID: 25917631].

  • 免疫印迹

    • WB result of NY-ESO-1 Rabbit mAb 
      Primary antibody: NY-ESO-1 Rabbit mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: HT-1080 whole cell lysate 20 µg
      Lane 3: TT whole cell lysate 20 µg
      Lane 4: A375 whole cell lysate 20 µg
      Negative control: Jurkat whole cell lysate 
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 18kDa
      Observed MW: 20kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human seminoma. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.