12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:20000 |
| IHC-P | 1:5000 |
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
WB result of Human IgG3 Rabbit mAb
Primary antibody: Human IgG3 Rabbit mAb at 1/1000 dilution
Lane 1: IgG1 FC recombinant protein lysate 10 ng
Lane 2: IgG2 FC recombinant protein lysate 10 ng
Lane 3: IgG3 FC recombinant protein lysate 10 ng
Lane 4: IgG4 FC recombinant protein lysate 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: IgG1 FC、IgG2 FC、IgG4 FC: 27 kDa; IgG3 FC: 33 kDa
Observed MW: IgG3 FC: 36 kDa
WB result of Human IgG3 Rabbit mAb
Primary antibody: Human IgG3 Rabbit mAb at 1/20000 dilution
Lane 1: Human serum lysate 5 μg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 58 kDa
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG3 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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