12 months from date of receipt / reconstitution, 4 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| IF | 1:1000 |
| ICFCM | 1:200 |
| ICC | 1:1000 |
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
SDT-132_alpha smooth muscle Actin (Alexa Fluor® 647)_FC_1
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody at 1/200 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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