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Akt (pan) Recombinant Rabbit mAb (SDT-R169)

Akt (pan) Recombinant Rabbit mAb (SDT-R169)

货号: S0B0114
价格: 600
规格: 25ul
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    AKT1 + AKT2 + AKT3

  • 分子别名

    N/A

  • 免疫原

    N/A

  • 细胞定位

    Cell membrane, Cytoplasm, Nucleus

  • Accession

    P31749, Q9Y243, P31751

  • 克隆号

    SDT-R169

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标签

    N/A

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500-1:2000
IP 1:50
背景介绍

  • Akt is a family of three serine-threonine kinases, Akt1, Akt2, and Akt3 [PMID: 28115590]. Akt is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. The Akt can be activated by a wide range of growth signals and the biochemical mechanisms leading to Akt activation are well defined [PMID: 31173856].

  • 免疫印迹

    • WB result of Akt (pan) Rabbit mAb 
      Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60kDa
      Observed MW: 60kDa

    • WB result of Akt (pan) Rabbit mAb 
      Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60kDa
      Observed MW: 60kDa

    • WB result of Akt (pan) Rabbit mAb 
      Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60kDa
      Observed MW: 60kDa

  • 免疫沉淀

    • Akt (pan) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Akt (pan) in 0.4 mg MCF-7 whole cell lysate.
      Western blot was performed on the immunoprecipitate using Akt (pan) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: MCF-7 whole cell lysate 20 µg (Input)
      Lane 2: Akt (pan) Rabbit mAb IP in MCF-7 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in MCF-7 whole cell lysate
      Predicted MW: 60 kDa
      Observed MW: 60 kDa


  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Akt (pan) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Akt (pan) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Akt (pan) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.