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alpha smooth muscle Actin Recombinant Mouse mAb (SDT-R142)

alpha smooth muscle Actin Recombinant Mouse mAb (SDT-R142)

货号: S0B0102
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    ALPHA SMOOTH MUSCLE ACTIN

  • 分子别名

    Alpha-actin-2, ACTA2, SMA

  • 免疫原

    N/A

  • 细胞定位

    Cytoplasm, Cytoskeleton

  • Accession

    P62736

  • 克隆号

    SDT-R142

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG2a

  • 应用

    ICFCM, IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.4 mg/ml

  • 标签

    N/A

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000-1:4000
IHC-P 1:4000
ICC 1:100
ICFCM 1:400
IP 1:40
背景介绍

  • α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

  • 免疫印迹

    • WB result of alpha smooth muscle Actin mouse mAb 
      Primary antibody: alpha smooth muscle Actin mouse mAb at 1/4000 dilution
      Lane 1: mouse skeletal muscle lysate 5 µg
      Lane 2: mouse stomach lysate 5 µg
      Negative control: mouse skeletal muscle lysate
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42kDa
      Observed MW: 42kDa

    • WB result of alpha smooth muscle Actin mouse mAb
      Primary antibody: alpha smooth muscle Actin mouse mAb at 1/1000 dilution
      Lane 1: NIH/3T3  whole cell lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42kDa
      Observed MW: 42kDa

  • 流式分析

    • Flow cytometric analysis of HeLa cells labelling alpha smooth muscle Actin antibody at 1/400 (0.1 μg) dilution/ (red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • alpha smooth muscle Actin Mouse mAb at 1/40 dilution (1 µg) immunoprecipitating alpha smooth muscle Actin in 0.4 mg NIH/3T3 whole cell lysate.
      Western blot was performed on the immunoprecipitate using alpha smooth muscle Actin Mouse mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: NIH/3T3 whole cell lysate 20 µg (Input)
      Lane 2: alpha smooth muscle Actin Mouse mAb IP in NIH/3T3 whole cell lysate
      Lane 3: Mouse monoclonal IgG2a IP in NIH/3T3 whole cell lysate
      Predicted MW: 42 kDa
      Observed MW: 42 kDa


  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI. Counterstain with tubulin (red).