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Invivo anti-mouse PD-1 Recombinant mAb (RMP1-14)

Invivo anti-mouse PD-1 Recombinant mAb (RMP1-14)

货号: S0B0101
价格: 700
规格: 1mg
介绍: -
其他: -
产品规格
  • 宿主来源

    Rat
  • 抗原名称

    PD-1
  • 分子别名

    Programmed cell death protein 1, mPD-1, CD279
  • 免疫原

    N/A
  • 细胞定位

    Cell membrane
  • Accession

    Q02242
  • 克隆号

    RMP1-14
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    Rat IgG2a, κ
  • 同型对照

    S0B0932
  • 应用

    In vivo blocking of PD-1/PD-L signaling, SPR, ICC, WB
  • 反应种属 ?

    Ms
  • 纯化方式

    Protein G
  • 浓度

    5 mg/ml
  • 内毒素含量

    <1EU/mg
  • 标签

    N/A
  • 性状

    Liquid
  • 缓冲体系

    PBS pH7.4, containing no preservative

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;

    -20 °C for 3 months under sterile conditions; 

    -80 °C for 24 months under sterile conditions.

    Please avoid repeated freeze-thaw cycles.

稀释度
应用 稀释度
WB 1:250
SPR 2000nM-31.25nM
ICC 1:500
背景介绍
  • Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of T and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells).

  • 验证数据

    • Anti-His antibody Immobilized on CM5 Chip captured PD1 His Tag, Mouse, can bind PD-1 Recombinant Rat mAb ( RMP1-14 ) with an affinity constant of 0.238 μM as determined in SPR assay.

  • 免疫印迹

    • WB result of PD-1 Rat mAb 
      Primary antibody: PD-1 Rat mAb at 1/250 dilution
      Lane 1:PD-1 Fc Chimera, Mouse (recombinant protein) lysate 1 μg
      Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 58~70 kDa
      Observed MW: 58~70 kDa

  • 免疫细胞化学

    • ICC shows positive staining in EL4.IL2 cells. Anti-PD-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • Negative control:ICC shows negative staining in NIH/3T3 cells. Anti-PD-1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).