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Lambda Light chain Recombinant Rabbit mAb (SDT-326-22)

Lambda Light chain Recombinant Rabbit mAb (SDT-326-22)

货号: S0B0086
价格: 600
规格: 25ul
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Lambda Light chain

  • 分子别名

    Immunoglobulin lambda constant 1, Ig lambda chain C region MGC, Ig lambda-1 chain C region

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted, Cell membrane

  • Accession

    P0CG04

  • 克隆号

    SDT-326-22

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000-1:5000
IHC-P 1:5000
ICC 1:2000
背景介绍

  • There are two types of light chains: kappa and lambda chains. The ratio or proportion between the kappa and lambda light chains indicates an excess production of one chain over the other, and therefore can be used as an indication of disease progression or remission.

  • 免疫印迹

    • WB result of Lambda Light chain Rabbit mAb Primary antibody: Lambda Light chain Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: Ramos whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 25kDa Observed MW: 27kDa
    • WB result of Lambda Light chain Rabbit mAb Primary antibody: Lambda Light chain Rabbit mAb at 1/5000 dilution Lane 1: human serum lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 25kDa Observed MW: 25kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Lambda Light chain antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human colon. Anti-Lambda Light chain antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Lambda Light chain antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in Ramos cells. Anti-Lambda Light chain antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
    • Negative control: ICC shows negative staining in Jurkat cells. Anti-Lambda Light chain antibody was used at 1/2000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).