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STING Recombinant Rabbit mAb (SDT-R150)

STING Recombinant Rabbit mAb (SDT-R150)

货号: S0B0084
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    STING

  • 分子别名

    Hsting, ERIS, hMITA, Transmembrane protein 173

  • 免疫原

    N/A

  • 细胞定位

    Endoplasmic reticulum membrane, Cytoplasm, Mitochondrion outer membrane, Cell membrane

  • Accession

    Q86WV6

  • 克隆号

    SDT-R150

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
IP 1:50
背景介绍

  • Mediator of IRF3 activation (MITA, also known as stimulator of interferon genes (STING) and endoplasmic reticulum interferon stimulator (ERIS)) is an ER-associated protein that senses cellular and bacterium-derived cyclic dinucleotide (CDN), leading to induction of type-I interferons (IFNs) and innate immune responses against viruses and bacteria [PMID: 36193584].

  • 免疫印迹

    • WB result of STING Rabbit mAb Primary antibody: STING Rabbit mAb at 1/1000 dilution Lane 1: THP-1 whole cell lysate 20 µg Lane 2: HaCaT whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42kDa Observed MW: 30, 37kDa

  • 免疫沉淀

    • STING Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating STING in 0.4 mg HL-60 whole cell lysate.
      Western blot was performed on the immunoprecipitate using STING Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HL-60 whole cell lysate 20 µg (Input)
      Lane 2: STING Rabbit mAb IP in HL-60 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HL-60 whole cell lysate
      Predicted MW: 42 kDa
      Observed MW: 37 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human colon. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human spleen. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human prostate. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.