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S-RMab® Kappa light chain Recombinant Rabbit mAb (SDT-R130)

S-RMab® Kappa light chain Recombinant Rabbit mAb (SDT-R130)

货号: S0B0083
价格: 600
规格: 25ul
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Kappa light chain

  • 分子别名

    N/A

  • 免疫原

    N/A

  • 克隆号

    SDT-R130

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
背景介绍

  • There are two types of light chains: kappa and lambda chains. The ratio or proportion between the kappa and lambda light chains indicates an excess production of one chain over the other, and therefore can be used as an indication of disease progression or remission.

  • 免疫印迹

    • WB result of Kappa light chain Rabbit mAb Primary antibody: Kappa light chain Rabbit mAb at 1/1000 dilution Lane 1: Human serum lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 12kDa Observed MW: 24kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Kappa light chain antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human spleen. Anti-Kappa light chain antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human colon. Anti-Kappa light chain antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Kappa light chain antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Kappa light chain antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.