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C4d Recombinant Rabbit mAb (SDT-330-2)

C4d Recombinant Rabbit mAb (SDT-330-2)

货号: S0B0082
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    C4d

  • 分子别名

    N/A

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted

  • Accession

    P0C0L4

  • 克隆号

    SDT-330-2

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.125 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:2500
IHC-P 1:1000
背景介绍

  • Complement component 4 (C4), in humans, is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system. It serves a number of critical functions in immunity, tolerance, and autoimmunity with the other numerous components. Furthermore, it is a crucial factor in connecting the recognition pathways of the overall system instigated by antibody-antigen (Ab-Ag) complexes to the other effector proteins of the innate immune response.C4d is a degradation product of complement activator. If C4d is high in the tumor of patients, its survival period will be reduced; Complement fragment C4d can be used as a biomarker for early diagnosis and prognosis prediction of lung cancer patients.

  • 免疫印迹

    • WB result of C4d Rabbit mAb Primary antibody: C4d Rabbit mAb at 1/2500 dilution Lane 1: T47D whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Negative control: T47D whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 193kDa Observed MW: 210kDa Exposure time: 90s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human kidney. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human liver. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.