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CD47 Recombinant Rabbit mAb (SDT-295-33)

CD47 Recombinant Rabbit mAb (SDT-295-33)

货号: S0B0081
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD47

  • 分子别名

    Leukocyte surface antigen CD47, IAP, MER6

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    Q08722

  • 克隆号

    SDT-295-33

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Or

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000
IHC-P 1:2000-1:4000
背景介绍

  • The transmembrane protein, CD47, is recognized as an important innate immune checkpoint, and CD47-targeted drugs have been in development with the aim of inhibiting the interaction between CD47 and the regulatory glycoprotein SIRPα, for antitumor immunotherapy. Further, CD47 mediates other essential functions such as cell proliferation, caspase-independent cell death (CICD), angiogenesis and other integrin-activation-dependent cell phenotypic responses when bound to thrombospondin-1 (TSP-1) or other ligands. Mounting strategies that target CD47 have been developed in pre-clinical and clinical trials, including antibodies, small molecules, siRNAs, and peptides, and some of them have shown great promise in cancer treatment [PMID: 35931392].

  • 免疫印迹

    • WB result of CD47 Rabbit mAb Primary antibody: CD47 Rabbit mAb at 1/1000 dilution Lane 1: HepG2 whole cell unboiled RIPA lysate 20 µg Lane 2: Jurkat whole cell unboiled RIPA lysate 20 µg Lane 3: OVCAR-3 whole cell unboiled RIPA lysate 20 µg Negative control: HepG2 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 35kDa Observed MW: 40~60kDa Exposure time: 20s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD47 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CD47 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.