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HSP60 Recombinant Rabbit mAb (Cy5 conjugate) (SDT-R012)

HSP60 Recombinant Rabbit mAb (Cy5 conjugate) (SDT-R012)

货号: S0B0077
价格: 480
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    HSP60

  • 分子别名

    60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein

  • 免疫原

    N/A

  • 细胞定位

    Mitochondrion Matrix

  • Accession

    P10809

  • 克隆号

    SDT-R012

  • 抗体类型

    Recombinant mAb

  • 应用

    ICC

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Cy5

  • 性状

    Liquid

  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied.

稀释度
应用 稀释度
ICC 1:100
背景介绍

  • HSP60, also known as chaperonins (Cpn), is a family of heat shock proteins originally sorted by their 60kDa molecular mass. They prevent misfolding of proteins during stressful situations such as high heat, by assisting protein folding. HSP60 belongs to a large class of molecules that assist protein folding, called molecular chaperones.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-HSP60 (Cy5 conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
    • ICC shows positive staining in NIH/3T3 cells. Anti-HSP60 (Cy5 conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
    • ICC shows positive staining in C6 cells. Anti-HSP60 (Cy5 conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).