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alpha smooth muscle Actin Recombinant Rabbit mAb (Cy5 Conjugate) (SDT-132-63)

alpha smooth muscle Actin Recombinant Rabbit mAb (Cy5 Conjugate) (SDT-132-63)

货号: S0B0076
价格: 1056
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    alpha smooth muscle Actin (SMA)
  • 分子别名

    Alpha-actin-2,ACTA2
  • 免疫原

    Synthetic Peptide
  • Accession

    P62736
  • 克隆号

    SDT-132-63
  • 抗体类型

    Recombinant mAb
  • 应用

    IF
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    2 mg/ml
  • 标记

    Cy5
  • 性状

    Liquid
  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied.

稀释度
应用 稀释度
IF 1:100
背景介绍
  • α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in non-muscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

  • 免疫组化

    • IF shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
    • IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
    • IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
    • IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.