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CD7 Recombinant Rabbit mAb (SDT-R165)

CD7 Recombinant Rabbit mAb (SDT-R165)

货号: S0B2171
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD7

  • 分子别名

    T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41

  • 免疫原

    N/A

  • 细胞定位

    Membrane

  • Accession

    P09564

  • 克隆号

    SDT-R165

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500
IHC-P 1:1000
ICC 1:125
IP 1:25
背景介绍

  • CD7 is a single-domain Ig superfamily molecule expressed on human T and NK cells, as well as on cells in the early stages of T, B, and myeloid cell differentiation. CD7 is highly expressed on malignant immature T cells and is generally absent on malignant mature T cells [PMID: 10530432]. CD7 is Lymphoid marker, which expressed in 30% of AML cases and linked with poor prognosis in myeloid malignancies [PMID: 21148082].

  • 免疫印迹

    • WB result of CD7 Rabbit mAb Primary antibody: CD7 Rabbit mAb at 1/500 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 25.4kDa Observed MW: 37 kDa

  • 免疫沉淀

    • CD7 Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating CD7 in 0.4 mg Jurkat whole cell lysate.
      Western blot was performed on the immunoprecipitate using CD7 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: Jurkat whole cell lysate 50 µg (Input)
      Lane 2: CD7 Rabbit mAb IP in Jurkat whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
      Predicted MW: 25.4 kDa
      Observed MW: 37 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human spleen. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human thymus. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human stomach. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in Jurkat cells. Anti-CD7 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
    • Negative control: ICC shows negative staining in Raji cells. Anti-CD7 antibody was used at 1/125 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).