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GPA33 Recombinant Rabbit mAb (SDT-248-76)

GPA33 Recombinant Rabbit mAb (SDT-248-76)

货号: S0B2168
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GPA33

  • 分子别名

    Cell surface A33 antigen, Glycoprotein A33

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Membrane

  • Accession

    Q99795

  • 克隆号

    SDT-248-76

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500
IHC-P 1:2000
背景介绍

  • The A33 cell-surface differentiation antigen is a glycoprotein that belongs to the immunoglobulin superfamily [PMID: 9012807]. This antigen is expressed in >95% of primary and metastatic colon cancer cells, but is absent in most normal and tumor cell types [PMID: 9245713, PMID: 16000578]. Although the exact function of this glycoprotein is unknown, antibodies developed against the A33 antigen bind to tumor cells and exhibit prolonged retention in the tumor tissue [PMID: 8656247].

  • 免疫印迹

    • WB result of GPA33 Rabbit mAb 
      Primary antibody: GPA33 Rabbit mAb at 1/500 dilution
      Lane 1: HT-29 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa 
      Observed MW: 50 kDa
      Exposure time: 180 s(This blot was developed with high sensitivity substrate)

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.