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Tau Recombinant Mouse mAb (SDT-R145)

Tau Recombinant Mouse mAb (SDT-R145)

货号: S0B0063
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    Tau

  • 分子别名

    Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

  • 免疫原

    N/A

  • 细胞定位

    Cell membrane, Cytoplasm, Secreted

  • Accession

    P10636

  • 克隆号

    SDT-R145

  • 抗体同种型

    IgG1

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000
IHC-P 1:2000
背景介绍

  • Tau is a microtubule-associated protein for which the physiological functions remain a topic of vigorous investigation. Additionally, tau is a central player in the pathogenesis of several diseases such as Alzheimer’s disease and several frontotemporal dementias. A critical variable to understanding tau in physiological and disease contexts is its normal localization within cells of the adult CNS.

  • 免疫印迹

    • WB result of Tau mouse mAb Primary antibody: Tau mouse mAb at 1/1000 dilution Lane 1: mouse liver lysate 20 µg Lane 2: mouse brain lysate 20 µg Negative control: mouse liver lysate Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50~65 kDa Observed MW: 50~65 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.