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CGA/HCG-α Recombinant Rabbit mAb (SDT-227-66)

CGA/HCG-α Recombinant Rabbit mAb (SDT-227-66)

货号: S0B2161
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CGA/HCG-α

  • 分子别名

    Glycoprotein hormones alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted

  • Accession

    P01215

  • 克隆号

    SDT-227-66

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:1000
背景介绍

  • Human chorionic gonadotropin (HCG) is a glycoprotein hormone consisting of two noncovalently bonded subunits, alpha and beta [PMID: 68892], and is normally synthesized by placental trophoblasts during pregnancy. The alpha subunit of hCG is identical to the alpha subunit of 3 hormones synthesized by the anterior pituitary gland but the beta subunit of each of these hormones is unique and confers biological specificity. Among the beta subunits, hCGbeta and Lhbeta are vey homologous such that both hormones bind to the LH receptor.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-CGA/HCG-α antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.