12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:250-1:500 |
| WB | 1:1000 |
| IP | 1:50 |
| ICFCM | 1:500 |
| ICC | 1:500 |
Transitions through the cell cycle are driven by cyclins and cyclin-dependent kinases (CDKs) [PubMed: 26658964]. Cyclins are the allosteric activators of cognate CDKs; their levels typically oscillate across the cell cycle, hence gaining the name cyclins. . The cyclin family shares a homologous N-terminal 100-amino acid motif referred as the cyclin box that has a highly conserved three-dimensional structure and provides the binding interface for the appropriate CDKs [PubMed: 8591034]. D cyclins, including cyclins D1, D2 and D3, form active complexes with either CDK4 or CDK6, which in turn phosphorylate the retinoblastoma protein (Rb) and drive G1 to S phase progression [PubMed: 7505440].
WB result of Cyclin D3 Rabbit mAb
Primary antibody: Cyclin D3 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Cyclin D3 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Cyclin D3 Rabbit mAb at 1/50 dilution (1µg) immunoprecipitating Cyclin D3 in 0.4mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Cyclin D3 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : HeLa whole cell lysate 10µg(input)
Lane 2 : Cyclin D3 Rabbit mAb IP in HeLa whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 33 kDa
Observed MW: 33 kDa
IHC shows positive staining in paraffin-embedded human pancreas. Anti-Cyclin D3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Cyclin D3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Cyclin D3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Cyclin D3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Cyclin D3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti-Cyclin D3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).
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