PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
The functions of the neuronal microtubule-associated protein Tau in the central nervous system are regulated by manifold posttranslational modifications at more than 50 sites. Tau in healthy neurons carries multiple phosphate groups, mostly in its microtubule assembly domain. Elevated phosphorylation and aggregation of Tau are widely considered pathological hallmarks in Alzheimer's disease (AD) and other tauopathies, triggering the quest for Tau posttranslational modifications in the disease context [PMID: 33892381]. Tau phosphorylation at Thr231 can be detected in cerebrospinal fluid and shows potential as a biomarker of neurofibrillary pathology in Alzheimer’s disease [PMID: 17012293].
WB result of Tau(phospho T231) Rabbit mAb
Primary antibody: Tau(phospho T231) Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 5 µg
Lane 2: mouse brain lysate(phosphatase treated) 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50~70 kDa
Observed MW: 50~70 kDa
Exposure time: 60s
Tau (phospho T231) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Tau (phospho T231) in 0.4 mg mouse brain lysate.
Western blot was performed on the immunoprecipitate using Tau (phospho T231) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: mouse brain lysate 20 µg (Input)
Lane 2: Tau (phospho T231) Rabbit mAb IP in mouse brain lysate
Lane 3: Rabbit monoclonal IgG IP in mouse brain lysate
Predicted MW: 50~70 kDa
Observed MW: 50~70 kDa
Exposure time: 150 s（This blot was developed with high sensitivity substrate）
Dot blot result of Tau (phospho T231) Rabbit mAb
Lane 1: Tau (phospho T231) peptide(aa224~240)
Lane 2: Tau non-phospho peptide(aa224~240)
Primary antibody: Tau(phospho T231) Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Exposure time: 8s
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