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CD73 Recombinant Rabbit mAb (SDT-218-52)

CD73 Recombinant Rabbit mAb (SDT-218-52)

货号: S0B2139
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    CD73
  • 分子别名

    5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cell membrane
  • Accession

    P21589
  • 克隆号

    SDT-218-52
  • 抗体类型

    Rabbit mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:500-1:1000
WB 1:1000
背景介绍
  • CD73, also known as ecto-5′-nucleotidase (ecto-5′-NT, EC 3.1.3.5) is a glycosylphosphatidyl inositol (GPI)-anchored cell surface protein that is encoded by NT5E gene. CD73 is widely expressed on different tissues [PubMed: 9553767, PubMed: 18404475] and cell types including, but not limited to the subsets of T cells and B cells [PubMed: 2550543], endothelial cells [PubMed: 9015312, PubMed: 15358667] and epithelial cells [PubMed: 9169488].

  • 免疫印迹

    • WB result of CD73 Rabbit mAb                 
      Primary antibody: CD73 Rabbit mAb at 1/1000 dilution
      Lane 1: Raji whole cell lysate 20 µg
      Lane 2: HT-29 whole cell lysate 20 µg
      Lane 3: A431 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Negative control: Raji whole cell lysate   
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 63 kDa
      Observed MW: 70 kDa
      Exposure time: 120s

    • WB result of CD73 Rabbit mAb                 
      Primary antibody: CD73 Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 63 kDa
      Observed MW: 68 kDa
      Exposure time: 180s

    • WB result of CD73 Rabbit mAb                 
      Primary antibody: CD73 Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 63 kDa
      Observed MW: 70 kDa
      Exposure time: 180s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human liver. Anti-CD73 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-CD73 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.